The dataset contains macrozoobenthos data collected during the MN247 Cruise from 22 stations located in the Romanian Black Sea area, in 21-24 August 2023, in the framework PN23300101 Project. The main goal of the cruise was to develop the national thematic maps (geological/geophysical/biological) at a scale of 1:50,000 of the Romanian continental shelf of the Black Sea. From each station, one replicate were collected with the with Van-Veen grab (the grasping area - 0.125 m2). The sediment was preserved in buffered formalin 4% , then sieved through a 1 mm and 0.5 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (density and wet weight biomass per square meter) for 75 macrozoobenthic species and 2 supraspecific groups.