The dataset contains macrozoobenthos data collected during the MN219 Cruise from 18 stations and observations on benthic habitats from 35 stations located in the Romanian Black Sea area, in 4-16 June 2021, in the framework PN19200101 Project. The main goal of the cruise was to develop the national thematic maps (geological/geophysical/biological) at a scale of 1:50,000 of the Romanian continental shelf of the Black Sea. From each station, one replicate were collected with the with Van-Veen grab (the grasping area - 0.125 m2). The sediment was preserved in buffered formalin 4% , then sieved through a 1 mm and 0.5 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (density and wet weight biomass per square meter) for 83 macrozoobenthic species and 2 supraspecific groups.