The dataset contains macrozoobenthos data collected during the MN244 Cruise from 25 stations located in the Romanian Black Sea area, in 10-16 June 2023, in the framework PN23300103 Project. The main goal of the cruise was to assess the ecological state of the ecosystem in the Romanian part of the Black Sea. From each station, one replicate were collected with the with Van-Veen grab (the grasping area - 0.125 m2). The sediment was preserved in buffered formalin 4% , then sieved through a 1 mm and 0.5 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (density and wet weight biomass per square meter) for 97 macrozoobenthic species and 2 supraspecific groups.